Resumen

GUEVARA SALCEDO, Limber; ARANCIBIA PADILLA, Giorjan Arnulfo  y  OSINAGA RICO, Edil. Propagación in vitro de Corryocactus pulquinensis Cárdenas, Cleistocactus candelilla Cárdenas y Echinopsis huotii (F. Cels) Labour. subsp. huotti. Info INIAF [online]. 2013, vol.1, n.2, pp. 77-80. ISSN 2308-250X.

The methods for disinfection and in vitro culture were determined for 3 species of Cactaceae from the protected area "Jardin de Cactaceas de Bolivia (AP-JCB)": Corryocactus pulquinensis Cárdenas, Cleistocactus candelilla Cárdenas y Echinopsis huotii (F. Cels) Labour subsp. huotti. The disinfection was achieved applying solutions of 40% formaldehide for 30 minutes, 80% ethanol for 1 minute, benomyl for 30 minutes, 2% bleach for 15 minutes and rinsing with sterile distilled water. The development of shoots was achieved with BAP (3 ml·L^-1) and activated charcoal (1 g·L^-1) in a solution of MS medium(4.43 g·L^-1), citric acid (300 mg·L^-1), sucrose (30 g·L^-1), and agar (7 g·L^-1) at a pH between 5.5 and 5.8.