Resumen

AMARU CALZADA, ARIEL  y  UNIVERSIDAD MAYOR DE SAN ANDRES et al. EXTRACCION DE DNA CON PAPAINA. Ciencia y Medicina [online]. 2004, vol.5, pp. 08-10. ISSN 1816-2908.

They ABRIDGE Words you nail.- DNA, Papaina, Leucocitos presently work was carried out the extrac-tion of the DNA [genómico], for the classical method of extraction ([fenol cloroformo]), utilizing the [enzima papaina] ([latex] of the papaya) instead of the K [proteinasa], with the objective of economizing the costs and present like model of you/he/she/it practice of extraction of DNA in the practice of Bioquímica and Molecular Biology. The pattern of [leucocitos] was gotten [eritrocítica] of 5 [ml] of veined outlying blood for the [lisis]. The [pelet] of [leucocitos] was dissolved with 2 [ml] of TNE and 100 [ml] of SDS at 10% 1. 50 [ml] of [papaina] (gotten of the fruit was added natural papaya). The [digestión] stayed for 12 hours to 37 grades [centígrados], in bathroom [maria]. Subsequently, to the tube 2 [ml] was added of I fenol-chlorine-form; he/she/it/you mixed for 5 minutes even [homogenizar], and [centrifugó] to 2000 [rpm] for 5minutos. Subsequently the [sobrenadante] was trans-ferred to another tube of rehearsal of 5 [ml]. To this 2 [ml] of chlorine-formo-alcohol was added [isomalico]; he/she/it/you mixed for 5 minutes even [homogenizar] and then [centrifugó] to 2000 [rpm] for 5minutos. To lat-est east 3 [ml] of [etanol] was added absolute cold (maintained to- 20°C), observing the precipitate of DNA. [centrifugó] to 2000 [rpm] for 5 minutes. The [sobrenadante] was eliminated and was made to dry in the environment. The precipitate of DNA was dissolved in 50 [ml] of distilled water. He/she/it/you was carried out the [electroforesis] in gel of [agarosa] with this pattern, next to another pattern of DNA extracted with k [proteinasa] and another extracted without k [proteinasa] neither [papaina]. The presence of DNA with the method was demonstrated [papaina]

Palabras llave : DNA; Papaina; Leucocitos.