Resumen

RIOS M., Neida; TERRAZAS SILES, Luis Enrique  y  ALVAREZ ALIAGA, Maria Teresa. Production of exoxilanolitic enzymes by immobilisation of FT3 strand extremophilus bacteria in calcium alginate. BIOFARBO [online]. 2009, vol.17, n.1, pp. 22-29. ISSN 1813-5363.

Xylanases are exoglucanases capable of producing xylane. These enzymes can be applied in several industrial processes such as agricultural residue treatment to obtain sugars which are later fermented to produce ethanol. However, this process requires stabilization at high temperatures; therefore there is great interest in the study of thermophilus micro organisms. This study focuses on the production of xylanases using calcium alginate for immobilisation of the FT3 thermophilus anaerobic bacteria, which was isolated from the andean region of Bolivia. The results showed that when the viscosity of the calcium alginate was 1%, a xylanolytic activity of 3 [IU/mL] was achieved. In batch cultures, the best immobilisation was achieved with high viscosity calcium alginate of at 2%, improving the enzymatic activity to 4.5 [IU/mL]. When a continuous system of immobilized cells was used in order to recover the enzyme, the enzymatic activity was increased to 15 [IU/mL]. When NH₄Cl was replaced by yeast extract as a source of nitrogen in the medium, the enzymatic activity was improved to 23, 5 [IU/mL] in the bioreactor.

Palabras llave : xylanases; thermophile; immobilisation; bioreactor.