Resumen

REYES GRATEROL, Elbert Oberto; DUGARTE QUINTERO, Leonardo Yhan Paolo; COVILLA HERNANDEZ, Erick Daniel  y  DIAZ SANABRIA, Corina Elena. Variant of the Ammoniacal Silver Nitrate Staining to emphasize dense plaques (Fibronexus) of Myofibroblast. SCIENTIFICA [online]. 2017, vol.15, n.1, pp. 7-12. ISSN 1813-0054.

Abstract Introduction: the use of silver stainings were developed in the XIX century, at the beginning were used to stain nervous tissues, and had a major role in the postulate of cellular theory as a biological dogma. After, It was used in connective tissue, but it have never been used in identification of myofibroblasts. Connective tissue cells with many functions and related with fibrosis. Objective: To determine the usefulness of a variant of the ammoniacal silver nitrate technique in selective staining of the fibronexus, an ultrastructural characteristic of myofibroblasts, in the renal capsule of Wistar rats. Materials and methods: Immunohistochemistry and electron microscopy were used to determine the presence of myofibroblasts in the renal capsule of Wistar rats. Finally, the variant of the ammoniacal silver nitrate technique. Results: the argyrophil plaques were found in myofibroblasts of the renal capsule which correspond to those ones in immunohistochemistry and electron microcscopy. Conclusions: the variant of the ammoniacal silver nitrate technique is useful in the selective staining of subplasmalemmal dense plaques in myofibroblastic morphology. However, it is still necessary to clarify its basis and the testing of the technique in other tissues.

Palabras llave : Silver Staining; Argentaífin; Argyrophil; Myofibroblasts; Fibronexus.