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Revista SCientifica
versión impresa ISSN 1813-0054
Resumen
R, Amaru; A, Rambaldi y A, Biondi. CLONACIÓN DEL GEN 633 Y CARACTERIZACIÓN MOLECULAR DE UNA NUEVA DELESIÓN SIL- 633. SCIENTIFICA [online]. 2008, vol.6, n.1, pp. 11-15. ISSN 1813-0054.
During the screening with southern blot for Tal 1 deletion, 633 patient presented a band of 5.5 kb, differently to described by previous studies. 3 RACE was identified two new sequences joined to exon 1b of the Sil gene: 633 gene isoform A and 633 isoform B, both genes were amplified from a Library-cDNA. L-DNA-PCR and Nested L-DNA-PCR, was determined the new break point and the new deletion Sil-633. The location of the 633 gene was determined on the locus 1p32.
Palabras llave : Sil, Tal1, 633, T-ALL, gene, deletion, cloning.