Abstract

ECHENIQUE QUEZADA, Marco Antonio  and  HUANCA CARDOZO, Wilfredo. Evaluation of antibiotics and fungicides in the in vitro introduction of banana (Musa acuminata) at the Sapecho Experimental Station. Apthapi [online]. 2022, vol.8, n.3, pp. 10-17. ISSN 0102-0304.

ABSTRACT: Decontamination of explants is a requirement for the efficiency of introduction and establishment of in vitro banana (Musa acuminata) explants. The work was carried out at the Plant Biotechnology Laboratory of the Sapecho Experimental Station, located in the municipality of Palos Blancos in the department of La Paz, with the objective of evaluating the efficiency of three antibiotics and three fungicides in the introduction and establishment of the banana (Musa acuminata) Grand Nain variety. The antibiotics used were Erythromycin, Tetracycline and Chloramphenicol, while the fungicides used were Benlate, Ridomil and Cuprocol in different doses. The three antibiotics were added to the introduction culture medium, while the fungicides were applied by submerging the explants before planting in the culture medium. For data analysis, a completely randomized design (CRD) was used, with nine treatments and three replicates. For the comparison of means, Fisher's test was applied at 5% probability. The variables evaluated were: total contamination and explant survival. After 45 days of in vitro culture, there were highly significant effects on fungal contamination (mean 33.3%) and bacterial contamination (48.1%). In the survival variable (mean 46%), there were significant differences inthefungicidexantibiotic interaction. The useoftheantibiotic Erythromycin in combination with the fungicide Cuprocol inthe introduction and establishment of banana allowsthe reductionof losses dueto bacterial and fungal contamination. In addition, according to the results observed, treatments T1 and T4 presented the highest percentage of contamination (77.8%), and the treatment with the highest percentage of survival was T6 (100%)

Keywords : Banana; Antibiotics; Fungicides; Establishment; in vitro.